General description

A lipophilic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles. Also useful in studying endocytosis and identifying cell membrane boundaries. The compound is non-fluorescent in aqueous solution but becomes fluorescent when incorporated into the plasma membrane. Reversible, photostable, and does not diffuse out of internalized vesicles. Can also be used in combination with other green fluoroprobes for double-labeling experiments.

A lipophilic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles.

A lipophilic, nontoxic, water-soluble styrylpyridinium red fluoroprobe that is useful for staining active recycling synaptic vesicles. Also useful for studying endocytosis, membrane turnover, and identifying cell membrane boundaries. This compound is non-fluorescent in aqueous solution, but becomes fluorescent when incorporated into the plasma membrane. Reversible, photostable material that does not diffuse out of internalized vesicles. Can also be used in combination with the SynaptoGreen^™ (Cat. No. 574798) to image plasma membranes independently in two colors. Excitation max.: 538-548 nm; emission max.: ~735 nm.

Legal Information

SynaptoRed is a trademark of Biotium, Inc.

SynaptoGreen is a trademark of Biotium, Inc.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Durand, J., et al. 2000. Neuroscience97, 11.Rouze, N.C. and Schwartz, E.A. 1998. J. Neurosci.18, 8614.Vida, T.A. and Emr, S.D. 1995. J. Cell Biol.128, 779.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)